Peptide based biomolecules have been known to be an important component of the innate immune systems of a wide variety of animals, plants and bacteria.1 Recently, short cationic peptide based mimics have proven to be consisting of antimicrobial properties individually.2,3 Also, different size and morphology based gold/silver nanoparticles (AuNPs/AgNPs) has been reported using natural amino acids as reducing/capping agent.4 However, the combined effect of metallic nanoparticles and short peptides has not been explored yet for biomedical applications. The present work discusses the synthesis of short cationic peptide labeled AuNPs/AgNPs and their characterization using various spectroscopic and microscopic techniques. The synthesized peptide-nano conjugates were observed to possess potent efficacy against tested microbial strains. Notably, it is the first extensive study that discusses the conjugation of dipeptides with metallic nanoparticles at molecular level as most of the earlier works in this direction have been performed with proteins and larger peptides; thereby further highlighting the cost effectiveness of the present approach.