Most naturally occurring peptides have poor proteolytic stability, which limits their therapeutic application. Cyclotides are plant-derived cyclic peptides that resist proteolysis due to their highly constrained structure, comprising a head-to-tail cyclic backbone and three disulfide bonds that form a cystine-knotted core. This makes them potentially useful as scaffolds onto which peptide sequences (epitopes) can be grafted. We target delivery of cyclotides via conjugation to a nanobody to confer specificity. We chose VHH7, an alpaca-derived nanobody that targets murine Class II MHC class II molecules, for conjugation of cyclotides and examined their immunogenic properties upon delivery to antigen presenting cells. Two cyclotides, MCoTI-I and MCoTI-I with a HA-tag epitope (YPYDVPDYA) grafted in loop 6 (MCoTI-HA), were tested. A conjugation strategy using sortase A and strain-promoted click chemistry provides a straight-forward method for targeted delivery of cyclotides to antigen-presenting cells and could play a role in the development of peptide-based vaccines. More generally, these types of fusions might also be useful to direct cyclotides to other cell types, based on the specificity of the VHH fusion partner.