Widespread pain is noted in many patients with Chronic Fatigue Syndrome (ME/CFS), Fibromyalgia (FM) and Temporomandibular disorders (TMD). These conditions usually start as a localized condition and spread to a widespread pain condition with increasing illness duration. The aim of the presentation is to assess the changes in the various Omic assessments to evaluate the underlying mechanisms associated with the development of widespread pain. Data from several studies will be combined in the presentation. Patients and age/sex matched controls had clinical examinations, completed questionnaires, standard serum biochemistry, glucose tolerance tests, serum, urine and faecal metabolomes performed. Increases in pain distribution were associated with reductions in serum essential amino acids, urea, serum sodium and increases in serum glucose and the 24-hour urine volume however the biochemistry was different for each pain area. Regression modelling revealed potential acetylation and methylation defects in the pain subjects. These changes appear consistent with repeated minor inflammatory mediated alterations in body and kidney function resulting in essential amino acid deprivation and inhibition of protein synthesis and genetic translation within tissues.